Type
Recombinant
Description
IL-12 isapotentregulatorofcell-mediatedimmuneresponsesanditinducesIFN-γproductionbyNKandTcells.Itisproducedbyactivatedmonocytes/macrophagecells,Blymphocytesandconnectivetissue-typemastcells.AmongitsBIOLOGicalactivities,IL-12 promotesthegrowthandactivityofactivatedNK,CD4+andCD8+cells,andinducesthedevelopmentofIFN-γ-producingTh1cells.RecombinantHumanIL-12 isa75.0 kDaheterodimericglycoproteinconsistingofdisulfide-linked35 kDa(p35)and40 kDa(p40)subunits(503 totalaminoacidresidues).
Source
ChineseHamsterOvaryCells(CHO)
Purity
98%
BiologicalActivity
TheED50 wasdeterminedbytheinductionofIFN-ɣ;fromNKcellsco-stimulatedwithIL-18 is1.0 ng/ml,correspondingtoaspecificactivityof1×106 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein1×PBS,pH7.2–7.4 toaconcentrationof0.2 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C