Type
Recombinant
Description
IL-11 isamultifunctionalcytokineproducedbystromalcells,suchasfibroblasts,epithelialcellsandosteoclasts.Itisexpressedinawidevarietyoftissues,includingthymus,lung,bone,connectivetissueandcentralnervoussystem.IL-11 playsanimportantregulatoryroleinhematopoiesisbystimulatinggrowthofmyeloid,erythroidandmegakaryocyteProgenitorcells.Italsoregulatesbonemetabolism,inhibitsproductionofproinflammatorycytokines,andprotectsagainstgastromucosalinjury.RecombinantMurineIL-11 isa19.1 kDaproteinconsistingof179 aminoacidresidues.
AminoAcidSequence
MPGPPAGSPRVSSDPRADLDSAVLLTRSLLADTRQLAAQMRDKFPADGDHSLDSLPTLAMSAGTLGSLQLPGVLTRLRVDLMSYLRHVQWLRRAGGPSLKTLEPELGALQARLERLLRRLQLLMSRLALPQAAPDQPVIPLGPPASAWGSIRAAHAILGGLHLTLDWAVRGLLLLKTRL
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofmurineT11cellsis2.0 ng/ml,correspondingtoaspecificactivityof5×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
