Type
Recombinant
Description
IL-10 isanimmunosuppressivecytokineproducedbyavarietyofmammaliancelltypesincludingmacrophages,monocytes,Tcells,Bcellsandkeratinocytes.IL-10 inhibitstheexpressionofproinflammatorycytokinessuchasIL-1 andTNF-α.LikeIL-4,IL-10 enhanceshumoralimmuneresponsesandattenuatescell-mediatedimmunereactions.HumanIL-10 isactiveonmurinecells,butmurineIL-10 isinactiveonhumancells.RecombinantRatIL-10 isan18.7 kDaproteinof160 aminoacidresidues.
AminoAcidSequence
SKGHSIKGDNNCTHFPVSQTHMLRELRAAFSQVKTFFQKKDQLDNIVLTDSLLQDFKGYLGCQALSEMIKFYLVEVMPQAENHGPEIKEHLNSLGEKLKTLWIQLRRCHRFLPCENKSKAVEQVKNDFNKLQDKGVYKAMNEFDIFINCIEAYVTLKMKN
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentinhibitionofantigen-specificTcellproliferationis≤10ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
