Type
Recombinant
Description
IL-10 isanimmunosuppressivecytokineproducedbyavarietyofmammaliancelltypesincludingmacrophages,monocytes,Tcells,Bcellsandkeratinocytes.IL-10 inhibitstheexpressionofproinflammatorycytokinessuchasIL-1 andTNF-α.LikeIL-4,IL-10 enhanceshumoralimmuneresponsesandattenuatescell-mediatedimmunereactions.HumanIL-10 isactiveonmurinecells,butmurineIL-10 isinactiveonhumancells.RecombinantHumanIL-10 isan18.6 kDaproteinof161 aminoacidresidues.
AminoAcidSequence
MSPGQGTQSENSCTHFPGNLPNMLRDLRDAFSRVKTFFQMKDQLDNLLLKESLLEDFKGYLGCQALSEMIQFYLEEVMPQAENQDPDIKAHVNSLGENLKTLRLRLRRCHRFLPCENKSKAVEQVKNAFNKLQEKGIYKAMSEFDIFINYIEAYMTMKIRN
Source
E.coli
Purity
98%
BIOLOGicalActivity
Determinedbythedose-dependentco-stimulation(withhumanIL-4)ofMC/9cells.TheED50 wasfoundtobe≤2.0 ng/ml,correspondingtoaspecificactivityof≥5×105 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein5 mMSodiumPhosphate,pH7.2 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
