Biovendor/Insulin-Like Growth Factor-Binding Protein 7 Human E. coli/RD172422100/0.1 mg-免疫在线蚂蚁淘旗下平台

商品详细Biovendor/Insulin-Like Growth Factor-Binding Protein 7 Human E. coli/RD172422100/0.1 mg

Biovendor/Insulin-Like Growth Factor-Binding Protein 7 Human E. coli/RD172422100/0.1 mg

商品编号： RD172422100

品牌： Biovendor-Laboratorni

市场价： ¥7000.00

美元价： 4200.00

产地：美国(厂家直采)

公司：

产品分类：细胞因子

公司分类： cytokine

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Regulatorystatus:RUO
Type:Recombinant
Source:E.coli
Othernames:IBP-7,IGF-bindingprotein7,IGFBP-7,IGFBP-rP1,PGI2-stimulatingfactor,Prostacyclin-stimulatingfactor,Tumor-derivedadhesionfactor,TAF,IGFBP7,MAC25,PSF
Species:Human

Type

Recombinant

Description

Total265 AA.MW:27.6 kDa(calculated).UniProtKBacc.no.Q16270(Ser27-Leu282).N-terminalhis-tag(9 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

MKHHHHHHASSSDTCGPCEPASCPPLPPLGCLLGETRDACGCCPMCARGEGEPCGGGGAGRGYCAPGMECVKSRKRRKGKAGAAAGGPGVSGVCVCKSRYPVCGSDGTTYPSGCQLRAASQRAESRGEKAITQVSKGTCEQGPSIVTPPKDIWNVTGAQVYLSCEVIGIPTPVLIWNKVKRGHYGVQRTELLPGDRDNLAIQTRGGPEKHEVTGWVLVSPLSKEDAGEYECHASNSQGQASASAKITVVDALHEIPVKKGEGAEL

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis: >90%

SDS-PAGEGel

12 %SDS-PAGEseparationofHumanIGFBPr-P1:
1. M.W.Marker –14,21,31,45,66,97 kDa
2.Reducedandboiledsample,2.5 μg/lane
3.Non-reducedandnon-boiledsample,2.5 μg/lane

Endotoxin

<1.0 EU/μg

Formulation

Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mlsolutionin20 mMTrisbuffer,50 mMNaCl,pH7.5+5%(w/v)Threalose.

Reconstitution

Add200 µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.

品牌介绍

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。