Type

Recombinant

Description

TheInsulin/Relaxinstructuralfamilyofproteinsconsistsofatleastsevenmembers,eachcontaininganinsulin-likeheterodimericstructureoftwopolypeptidechainsheldtogetherbytwointer-chaindisulfidebonds.TheINSL5/INSL7hybridcontainstheβ-chainofINSL7(Relaxin-3),linkedtotheα-chainofINSL5.TheINSL5/INSL7hybridproteincanspecificallyactivatetheGprotein-coupledreceptors,GPCR135andGPCR142,butunlikeRelaxin-3,doesnotinducesignificantactivationoftheLGR7receptor.RecombinantHumanINSL5/INSL7hybridisaheterodimeric,5.2 kDaproteinconsistingofa27 aminoacidβ-chainlinkedtoa21 aminoacidα-chainbytwodisulfide bonds.

Source

E.coli

Purity

98%

BIOLOGicalActivity

Datanotavailable.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C