Type
Recombinant
Description
TheIGFsaremitogenic,polypeptidegrowthfactorsthatstimulatetheproliferationandsurvivalofvariouscelltypes,includingmuscle,bone,andcartilagetissueinvitro.IGFsarepredominantlyproducedbytheliver,althoughavarietyoftissuesproducetheIGFsatdistinctivetimes.TheIGFsbelongtotheInsulingenefamily,whichalsocontainsinsulinandrelaxin.TheIGFsaresimilartoinsulinbystructureandfunction,buthaveamuchhighergrowth-promotingactivitythaninsulin.IGF-IIexpressionisinfluencedbyplacentalactogen,whileIGF-I expressionisregulatedbygrowthhormone.BothIGF-I andIGF-IIsignalthroughthetyrosinekinasetypeI receptor(IGF-IR),butIGF-IIcanalsosignalthroughtheIGF-II/Mannose-6-phosphatereceptor.MatureIGFsaregeneratedbyproteolyticprocessingofinactiveprecursorproteins,whichcontainN-terminalandC-terminalpropeptideregions.RecombinantMurineIGF-I isa7.6 kDaglobularproteincontainingaminoacidsincluding3 intra-moleculardisulfide bonds.
AminoAcidSequence
GPETLCGAELVDALQFVCGPRGFYFNKPTGYGSSIRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPTKAA
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50wasdeterminedacellproliferationassayusingFDC-P1cellsis≤2.0 ng/ml,correspondingtoaspecificactivityof≥5×105units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
