Type
Recombinant
Description
TheIGFsaremitogenic,polypeptidegrowthfactorsthatstimulatetheproliferationandsurvivalofvariouscelltypes,includingmuscle,bone,andcartilagetissueinvitro.IGFsarepredominantlyproducedbytheliver,althoughavarietyoftissuesproducetheIGFsatdistinctivetimes.TheIGFsbelongtotheInsulingenefamily,whichalsocontainsinsulinandrelaxin.TheIGFsaresimilartoinsulinbystructureandfunction,buthaveamuchhighergrowth-promotingactivitythaninsulin.IGF-IIexpressionisinfluencedbyplacentalactogen,whileIGF-I expressionisregulatedbygrowthhormone.BothIGF-I andIGF-IIsignalthroughthetyrosinekinasetypeI receptor(IGF-IR),butIGF-IIcanalsosignalthroughtheIGF-II/Mannose-6-phosphatereceptor.MatureIGFsaregeneratedbyproteolyticprocessingofinactiveprecursorproteins,whichcontainN-terminalandC-terminalpropeptideregions.RecombinantHumanIGF-I andIGF-IIareglobularproteinscontaining70 and67 aminoacids,respectively,and3 intra-moleculardisulfidebonds.ThecalculatedmolecularweightofRecombinantHumanIGF-I is7.6 kDa.
AminoAcidSequence
GPETLCGAELVDALQFVCGDRGFYFNKPTGYGSSSRRAPQTGIVDECCFRSCDLRRLEMYCAPLKPAKSA
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 wasdeterminedbyacellproliferationassayusingFDC-P1cellsis≤2.0 ng/ml,correspondingtoaspecificactivityof≥5×105 units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
