Type
Recombinant
Description
IGF-BPscontrolthedistribution,functionandactivityofIGFsinvariouscelltissuesandbodyfluids.Currently,therearesevennamedIGF-BPsthatformhighaffinitycomplexeswithbothIGF-I andIGF-II.IGF-BP5isa28.6 kDa,cysteine-rich,secretedproteinproducedbyvascularsmoothmusclecells.ItisthemajorIGF-bindingproteinpresentinbonetissueandhelpspotentiatetheactionofIGF-I onsmoothmusclecells,fibroblasts,andosteoblasts.DatashowsthatIGFBP-5 actsasagrowthinhibitorandpro-apoptoticagentinbreastcancercells.IGFBP-5-overexpressingmiceshowanincreaseinneonatalmortality,reducedfemalefertility,whole-bodygrowthinhibition,andretardedmuscledevelopment.RecombinantHumanIGF-BP5isa28.6 kDaproteinconsistingof253 aminoacidresidues.
AminoAcidSequence
MLGSFVHCEPCDEKALSMCPPSPLGCELVKEPGCGCCMTCALAEGQSCGVYTERCAQGLRCLPRQDEEKPLHALLHGRGVCLNEKSYREQVKIERDSREHEEPTTSEMAEETYSPKIFRPKHTRISELKAEAVKKDRRKKLTQSKFVGGAENTAHPRIISAPEMRQESEQGPCRRHMEASLQELKASPRMVPRAVYLPNCDRKGFYKRKQCKPSRGRKRGICWCVDKYGMKLPGMEYVDGDFQCHTFDSSNVS
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 wasdeterminedbyitsABIlitytoinhibitIGF-IIinducedproliferationofMCF-7 is0.3 µg/mlinthepresenceof15 ng/mlofhumanIGF-II.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
