Biovendor/IGF-BP4 Human (BTI-Tn-5B1-4) Hi-5 Insect cells*/RBG10191020/20 µg-免疫在线蚂蚁淘旗下平台

商品详细Biovendor/IGF-BP4 Human (BTI-Tn-5B1-4) Hi-5 Insect cells*/RBG10191020/20 µg

Biovendor/IGF-BP4 Human (BTI-Tn-5B1-4) Hi-5 Insect cells*/RBG10191020/20 µg

商品编号： RBG10191020

品牌： Biovendor-Laboratorni

市场价： ¥0.00

美元价： 0.00

产地：美国(厂家直采)

公司：

产品分类：细胞因子

公司分类： cytokine

联系Q Q： 3392242852

电话号码： 4000-520-616

电子邮箱： info@ebiomall.com

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商品介绍

Regulatorystatus:RUO
Type:Recombinant
Source:(BTI-Tn-5B1-4)Hi-5Insectcells*
Othernames:Insulin-likeGrowthFactor-BindingProtein4,IBP-4,HT29-IGF-BP,coloncancercellgrowthinhibitor
Species:Human

Type

Recombinant

Description

IGF-BPscontrolthedistribution,functionandactivityofIGFsinvariouscelltissuesandbodyfluids.IGF-BP4isthemajorIGF-BPproducedbyosteoblasts,andisfoundintheepidermis,ovarianfollicles,andothertissues.IGF-BP4inhibitstheactivityofIGF-I andIGF-IIbybindinginamannerthatresultsintheformationofcomplexeswithreducedABIlitytosignalthroughcellsurfaceIGFreceptors.IGF-BP4caninhibitthegrowthofchickpelviscartilageandHT29colonadenocarcinomacellsbyblockingthemitogenicactionsofIGFs,andhasalsobeenshowntoreducecolonyformationbycolorectalcancercellsviaanIGF-independentpathway.TheBIOLOGicaleffectsofIGF-BP4canberegulatedbyPregnancyAssociatedPlasmaProteinA(PAPP-A),whichreducesIGF-BP4/IGFbindingaffinitybyproteolyticallycleavingIGF-BP4.ThemodulationofIGF-BP4activitybyPAPP-A isanimportantcomponentintheregulationofovarianfolliculogenesisandinthegrowthinhibitionofrespondingovariancancercells.RecombinantHumanIGF-BP4isa25.7 kDaproteinconsistingof237 aminoacidresiduesincluding,theIGF-BPdomainandthyroglobulintype-I domain.

AminoAcidSequence

DEAIHCPPCSEEKLARCRPPVGCEELVREPGCGCCATCALGLGMPCGVYTPRCGSGLRCYPPRGVEKPLHTLMHGQGVCMELAEIEAIQESLQPSDKDEGDHPNNSFSPCSAHDRRCLQKHFAKIRDRSTSGGKMKVNGAPREDARPVPQGSCQSELHRALERLAASQSRTHEDLYIIPIPNCDRNGNFHPKQCHPALDGQRGKCWCVDRKTGVKLPGGLEPKGELDCHQLADSFRE

Source

(BTI-Tn-5B1-4)Hi-5Insectcells*

Purity

95%

BiologicalActivity

DeterminedbyitsabilitytoinhibitIGF-I inducedproliferationofFDC-P1 cells.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

–20°C

品牌介绍

miREIA是一种基于免疫测定的新型miRNA定量方法，涉及从患者样品中分离出的miRNA与互补的生物素化DNA寡核苷酸探针杂交。然后通过固定有完美匹配的DNA / miRNA杂交体的微量滴定板固定的单克隆抗体捕获DNA / RNA杂交体。下一步是复制标准ELISA方案。该测定可在常规免疫测定设备上运行，与标准临床工作流程兼容，不需要扩增步骤，并且包括miRNA分析在内的结果可在不到三个小时的时间内获得。