Type

Recombinant

Description

IGF-BPscontrolsthedistribution,functionandactivityofIGFsinvariouscelltissuesandbodyfluids.Currently,therearesevennamedIGF-BPsthatformhighaffinitycomplexeswithbothIGF-I andIGF-II.IGF-BP2isacysteine-rich,secretedproteinproducedbybonecells,andismostabundantinthebrain.IGF-BP2hasbeenshowntoinhibitIGF-IIactioninhumanbreastandovariancarcinomacells.RecombinantHumanIGF-BP2isa31.5 kDaproteinconsistingof289 aminoacidresiduesincludingtheIGF-BPdomainandthyroglobulintype-I domain.

AminoAcidSequence

EVLFRCPPCTPERLAACGPPPVAPPAAVAAVAGGARMPCAELVREPGCGCCSVCARLEGEACQVYTPRCGQGLRCYPHPGSELPLQALVMGEGTCEKRRDAEYGASPEQVADNGDDHSEGGLVENHVDSTMNMLGGGGSAGRKPLKSGMKELAVFREKVTEQHRQMGKGGKHHLGLEEPKKLRPPPARTPCQQELDQVLERISTMRLPDERGPLEHLYSLHIPNCDKHGLYNLKQCKMSLNGQRGECWCVNPNTGKLIQGAPTIRGDPECHLFYNEQQEARGVHTQRMQ

Source

(BTI-Tn-5B1-4)Hi-5Insectcells*

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytoinhibitIGF-I inducedproliferationofFDC-P1 cells.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C