Type
Recombinant
Description
IGF-BPscontrolsthedistribution,functionandactivityofIGFsinvariouscelltissuesandbodyfluids.Currently,therearesevennamedIGF-BPsthatformhigh-affinitycomplexeswithbothIGF-I andIGF-II.IGF-BP1isa25.4 kDa,cysteine-rich,secretedproteinexpressedintheliver,deciduas,kidneys,andinamnioticfluid,whereitisthemostabundantIGF-BP.LevelsofIGF-BP1inserumarelowestafterfoodconsumption.IGF-BP1bindstobothIGF-I andIGF-IIwithequalaffinity.PhosphorylatedIGF-BP1hindersIGFactions,whereasnonphosphorylatedIGF-BP1isstimulatory.RecombinantHumanIGF-BP1isa25.4 kDaproteinconsistingof235 aminoacidresidues(Isoform A).
AminoAcidSequence
MAPWQCAPCSAEKLALCPPVSASCSEVTRSAGCGCCPMCALPLGAACGVATARCARGLSCRALPGEQQPLHALTRGQGACVQESDASAPHAAEAGSPESPESTEITEEELLDNFHLMAPSEEDHSILWDAISTYDGSKALHVTNIKKWKEPCRIELYRVVESLAKAQETSGEEISKFYLPNCNKNGFYHSRQCETSMDGEAGLCWCVYPWNGKRIPGSPEIRGDPNCQIYFNVQN
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 wasdeterminedbyitsABIlitytoinhibitIGF-I inducedproliferationofMCF-7 is0.5 µg/mlinthepresenceof6 ng/mlofhuman IGF-I.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
