Type

Recombinant

Description

IFNλ1,2,and3(alsoknownasIL-29,IL-28AandIL-28Brespectively)aredistantlyrelatedtotheIL-10 familyandinterferons.AllthreeIFN-lamBDasuseadistinctreceptorsystemcomposedofanIFN-λR1subunit(alsocalledCRF2–12)andIL-10R2subunit(alsocalledCRF2–14).Signalingthroughthisreceptorsysteminducesantiviraldefensessimilarto,butdistinctfrom,thatoftypeI interferons.RecombinantMurineIFN-λ2isa19.8 kDaproteincontaining175 aminoacidresidues.

AminoAcidSequence

MDPVPRATRLPVEAKDCHIAQFKSLSPKELQAFKKAKDAIEKRLLEKDMRCSSHLISRAWDLKQLQVQERPKALQAEVALTLKVWENMTDSALATILGQPLHTLSHIHSQLQTCTQLQATAEPKPPSRRLSRWLHRLQEAQSKETPGCLEDSVTSNLFRLLTRDLKCVASGDQCV

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytoactivateSTATfollowingreceptor-ligandinteraction.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C