Type
Recombinant
Description
IFNλ1,2,and3(alsoknownasIL-29,IL-28AandIL-28Brespectively)aredistantlyrelatedtotheIL-10 familyandinterferons.AllthreeIFN-lamBDasuseadistinctreceptorsystemcomposedofanIFN-λR1subunit(alsocalledCRF2–12)andIL-10R2subunit(alsocalledCRF2–14).Signalingthroughthisreceptorsysteminducesantiviraldefensessimilarto,butdistinctfrom,thanthatoftypeI interferons.RecombinantHumanIFN-λ2isa19.6 kDaproteincontaining174 aminoacidresidues.
AminoAcidSequence
PVARLHGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLKDCRCHSRLFPRTWDLRQLQVRERPMALEAELALTLKVLEATADTDPALVDVLDQPLHTLHHILSQFRACIQPQPTAGPRTRGRLHHWLYRLQEAPKKESPGCLEASVTFNLFRLLTRDLNCVASGDLCV
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoactivateSTATphosphorylationinanISRELuciferaseReporterAssayusinghumancoloncarcinomaCOLO205cells.TheexpectedED50 is0.3–0.5 ng/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.5–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
