Type
Recombinant
Description
IFN-γisanacid-lABIleinterferonproducedbyCD4andCD8Tlymphocytes,aswellasactivatedNKcells.IFN-γreceptorsarepresentinmostimmunecells,whichrespondtoIFN-γsignalingbyincreasingthesurfaceexpressionofclassI MHCproteins.ThispromotesthepresentationofantigentoT-helper(CD4+)cells.IFN-γsignalinginantigen-presentingcells,andantigen-recognizingBandTlymphocytes,regulatestheantigen-specificphasesoftheimmuneresponse.Additionally,IFN-γstimulatesanumberoflymphoidcellfunctions,includingtheanti-microbialandanti-tumorresponsesofmacrophages,NKcells,andneutrophils.HumanIFN-γisspecies-specificandisBIOLOGicallyactiveonlyinhumanandprimatecells.RecombinantratIFN-γisa15.6 kDaproteincontaining135 aminoacidresidues.
AminoAcidSequence
MQGTLIESLESLKNYFNSSSMDAMEGKSLLLDIWRNWQKDGNTKILESQIISFYLRLFEVLKDNQAISNNISVIESHLITNFFSNSKAKKDAFMSIAKFEVNNPQIQHKAVNELIRVIHQLSPESSLRKRKRSRC
Source
E.coli
Purity
98%
BiologicalActivity
TheED50asdeterminedbyacytopathicaffectinhibitionassaywithmurineL929cellschallengedwithEMCviruswas≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein10 mMSodiumPhosphate,pH8.0 toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
