Type
Recombinant
Description
IFN-γisanacid-lABIleinterferonproducedbyCD4andCD8Tlymphocytes,aswellasactivatedNKcells.IFN-γreceptorsarepresentinmostimmunecells,whichrespondtoIFN-γsignalingbyincreasingthesurfaceexpressionofclassI MHCproteins.ThispromotesthepresentationofantigentoT-helper(CD4+)cells.IFN-γsignalinginantigen-presenting,cellsandantigen-recognizingBandTlymphocytes,regulatestheantigen-specificphasesoftheimmuneresponse.Additionally,IFN-γstimulatesanumberoflymphoidcellfunctions,includingtheanti-microbialandanti-tumorresponsesofmacrophages,NKcells,andneutrophils.HumanIFN-γisspecies-specificandisBIOLOGicallyactiveonlyinhumanandprimatecells.RecombinantMurineIFN-γisa15.6 kDaproteincontaining134 aminoacidresidues.
AminoAcidSequence
MHGTVIESLESLNNYFNSSGIDVEEKSLFLDIWRNWQKDGDMKILQSQIISFYLRLFEVLKDNQAISNNISVIESHLITTFFSNSKAKKDAFMSIAKFEVNNPQVQRQAFNELIRVVHQLLPESSLRKRKRSRC
Source
E.coli
Purity
98%
BiologicalActivity
DeterminedbyitsabilitytoinhibittheproliferationofmurineWEHI-279 cells.TheexpectedED50is≤0.2 ng/ml,correspondingtoaspecificactivityof≥5×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstitutein5–10mMSodiumPhosphate,pH8.0 toaconcentrationof1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
