Type

Recombinant

AminoAcidSequence

MQDPYVKEAENLKKYFNAGHSDVADNGTLFLGILKNWKEESDRKIMQSQIVSFYFKLFKNFKDDQSIQKSVETIKEDMNVKFFNSNKKKRDDFEKLTNYSVTDLNVQRKAIHELIQVMAELSPAAKTGKRKRSQMLFQGRRASQ

Source

E.coli

Purity

98%

BIOLOGicalActivity

Assay#1:DeterminedbyitsABIlitytoinduceapoptosisinHeLacells.TheexpectedED50forthiseffectis5.0–10.0 ng/ml.Assay#2:TheED50wasdeterminedbyacytotoxicityassayusingHT-29 cellsis0.05 ng/ml,correspondingtoaspecificactivityof2×107units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C