Type

Recombinant

Description

Proteinsofthisfamilyplayanimportantroleininducingnon-specificresistanceagainstabroadrangeofviralinfections.Theyalsoaffectcellproliferationandmodulateimmuneresponses.Producedbyperipheralbloodleukocytesandlymphoblastoidcells,IFN-αisanacid-stablemoleculethatsignalsthroughIFN-α/βR,whichisalsousedbyIFN-β.BothIFNshavesimilaranti-viralactivityandregulateexpressionofMHCclassI antigens.IFN-αcontainsfourhighlyconservedcysteineresiduesthatformtwodisulfidebonds,oneofwhichisnecessaryforBIOLOGicalactivity.PeproTech’s Re­combinantHumanIFN-βisa20.0 kDaproteincontaining166 aminoacidresidues.Duetoglycosylation,IFN-βhasanapproximateMWof22.3 kDabasedonSDS-PAGEgelandMassSpectrometry.

AminoAcidSequence

MSYNLLGFLQRSSNFQCQKLLWQLNGRLEYCLKDRMNFDIPEEIKQLQQFQKEDAALTIYEMLQNIFAIFRQDSSSTGWNETIVENLLANVYHQINHLKTVLEEKLEKEDFTRGKLMSSLHLKRYYGRILHYLKAKEYSHCAWTIVRVEILRNFYFINRLTGYLRN

Source

ChineseHamsterOvaryCells(CHO)

Purity

95%

BiologicalActivity

Assay#1:MeasuredbyitsABIlitytoinduceapoptosisinHeLacells.TheexpectedED₅₀ forthiseffectis20–30ng/ml.Assay#2:DeterminedbyitsabilitytostimulatetheproliferationofhumanTF-1 cells.TheexpectedED₅₀ is≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×10⁷ units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C