Type

Recombinant

Description

I-309 isaCCchemokinethatsignalsthroughtheCCR8receptor.ItissecretedbyT-lymphocytes,monocytesandmastcells.I-309 chemoattractsmonocytesandTh4differentiatedT-cells,butnotneutrophils.HumanI-309 isactiveonmurinecells.RecombinanthumanI-309 isan8.5 kDaproteinconsistingof74 aminoacidresiduesthatincludesixcysteineresidues,ofwhichfourarethehighlyconservedcysteineresiduespresentinCCchemokines.UnlikesomeCCchemokines,whoseBIOLOGicallyactiveforminsolutionisanon-disulfide-linkeddimer,thebiologicallyactiveformofI-309 ismonomeric.

AminoAcidSequence

SKSMQVPFSRCCFSFAEQEIPLRAILCYRNTSSICSNEGLIFKLKRGKEACALDTVGWVQRHRKMLRHCPSKRK

Source

E.coli

Purity

98%

BiologicalActivity

DeterminedbyitsABIlitytochemoattracttotalhumanTcellpopulationusingaconcentrationrangeof10.0–100.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C