Type

Recombinant

Description

Chemerinisasecretedchemoattractantproteinthatcansignalthroughthechemokine-likereceptor-1(CMKLR1).Itisexpressedinvarioustissues,includingwhiteADIposetissue,andcirculatesinbloodasaninactive143 aminoacidprecursorprotein.BIOLOGicallyactiveChemerinisgeneratedbyproteolyticremovalofC-terminalresiduesbyseveralcirculatingproteases.ChemerinactsasachemoattractantforcellsexpressingtheCMKLR1receptor,whichincludescertaindendriticcells,macrophages,andadipocytes.RecombinantHumanChemerinisa15.6 kDaproteinconsistingof135 aminoacidresidues.

AminoAcidSequence

ELTEAQRRGLQVALEEFHKHPPVQWAFQETSVESAVDTPFPAGIFVRLEFKLQQTSCRKRDWKKPECKVRPNGRKRKCLACIKLGSEDKVLGRLVHCPIETQVLREAEEHQETQCLRVQRAGEDPHSFYFPGQFA

Source

E.coli

Purity

98%

BiologicalActivity

DeterminedbyitsABIlitytochemoattracthumanimmaturedendriticcellsusingaconcentrationrangeof1.0–100.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C