Type
Recombinant
Description
HVEMbelongstotheTNFReceptorsuperfamilyoftransmembraneproteins,andplaysaroleintheactivationofT-cellsandotherlymphocytes.Itisexpressedinvariouscellsandtissues,includingspleen,thymus,lung,macrophages,andT-cells.HVEMactivationinducesasignalingcascadethatresultsintheinductionoftranscriptionfactorsNF-κBandAP-1. LIGHT(TNFSF14)andTNF-β(TNFSF1)functionastheligandsforHVEM,whichcanalsobindspecificallytoherpessimplexvirusglycoproteinD.SolubleHVEMcanactasa“receptordecoy”resultingininhibitionoftheactivityoftheHVEMligands,LIGHTandTNF-β.RecombinanthumanHVEM-FcChimeraisa376 aminoacidfusionproteinthatcontainsanN-terminaldomaincorrespondingtotheextracellularregionofHVEM,andaC-terminaldomaincorrespondingtoresidues102 to330 ofhumanIgG1.ThecalculatedmolecularweightofRecombinantHumanHVEM-Fcis41.4 kDa.
AminoAcidSequence
LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKRSCDKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVDVSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVSNKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWESNGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLSLSPGK
Source
Hi-5Insectcells
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoneutralize0.25 ng/mlofhTNFβ;inducedcytotoxicityonmurineL929cells.TheexpectedED50 forthiseffectis1.3–1.9 µg/mlofHVEM-Fc.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
