Type

Active

Description

Total99 AA.MW:10.7 kDa(monomer),proteinactiveas dimer

AminoAcidSequence

PQFSLWKRPVVTAHIEGQPVEVLLDTGADDSIVAGIELGSNYSPKIVGGIGGFINTKEYKNVEIEVLNKRVRATIMTGDTPINIFGRNILASLGMSLNL

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis: >95%

SDS-PAGEGel

14%SDS-PAGEseparationofHumanHIV-2 Protease
1. M.W.Marker –10,20,30,40,60,80 kDa
2.reducedandheatedsample,2.5 μg/lane

Formulation

20 mMTris,20 mMMES,200 mMNaCl,10%glycerol,1 mMEDTA,0.5 mMDTT,0.05%PEG8000,pH7.0 –filtered(0.4 μm), frozen

Reconstitution

Defrostatambienttemperature.

Applications

Kineticstudies,Inhibitorscreening,Crystallography

Shipping

Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storeproteinat –80°C.Proteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Avoidrepeatedfreezing/thawingcycles.

QualityControlTest

SDSPAGEtodeterminepurityoftheprotein.
Activesitetitrationbytightlybindinginhibitor.

Note

Km=740 μMKcat=3 s-1 Kcat/Km=4.1 mM-1 s-1 withpeptidesubstrateATLNFPISPW