Type

Active

Description

Total 99 AA. MW: 10.8 kDa (monomer), protein active as dimer

Amino Acid Sequence

PQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIGGIGGFIKVRQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNF

Source

E. coli

Purity

Purity as determined by densitometric image analysis: >95%

SDS-PAGE Gel

14% SDS-PAGE separation of Human HIV-1 Protease
1. M.W. marker – 14, 21, 31, 45, 66, 97 kDa
2. reduced and heated sample, 2.5 μg/lane

Formulation

20 mM Tris, 20 mM MES, 200 mM NaCl, 1mM EDTA, 10% (v/v) glycerol, 0,05% 2-mercaptoethanol, pH 6.5 – filtered (0.4 μm), frozen

Reconstitution

Defrost at ambient temperature.

Applications

Kinetic studies, Inhibitor screening, Crystallography

Shipping

On ice. Upon receipt, store the product at the temperature recommended below.

Storage/Expiration

Store protein at –80°C. Protein remains stable until the expiry date when stored at –80°C. Avoid repeated freezing/thawing cycles.

Quality Control Test

SDS PAGE to determine purity of the protein.
Active site titration by tightly binding inhibitor.

Note

Km = 15.1 μM Kcat = 30 s-1 Kcat /Km = 1981 mM-1 s-1 with peptide substrate KARVF(NO2 )VRKA (F(NO2 ) … p-nitrophenylalanine)