Type
Recombinant
Description
Total398 AA.MW:43.4 kDa(calculated).UniProtKBacc.no.Q9UK79.397 AAofrecombinantHumanHerstatinandoneextraAA,N-terminalmethionin(highlighted).
AminoAcidSequence
MTQVCTGTDMKLRLPASPETHLDMLRHLYQGCQVVQGNLELTYLPTNASLSFLQDIQEVQGYVLIAHNQVRQVPLQRLRIVRGTQLFEDNYALAVLDNGDPLNNTTPVTGASPGGLRELQLRSLTEILKGGVLIQRNPQLCYQDTILWKDIFHKNNQLALTLIDTNRSRACHPCSPMCKGSRCWGESSEDCQSLTRTVCAGGCARCKGPLPTDCCHEQCAAGCTGPKHSDCLACLHFNHSGICELHCPALVTYNTDTFESMPNPEGRYTFGASCVTACPYNYLSTDVGSCTLVCPLHNQEVTAEDGTQRCEKCSKPCARGTHSLPPRPAAVPVPLRMQPGPAHPVLSFLRPSWDLVSAFYSLPLAPLSPTSVPISPVSVGRGPDPDAHVAVDLSRYEG
Source
E.coli
Purity
Purityasdeterminedbydensitometricimageanalysis:> 95%
SDS-PAGEGel
12%SDS-PAGEseparationofHumanHerstatin
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandheatedsample,2.5μg/lane
3.non-reducedandnon-heatedsample,2.5μg/lane
Endotoxin
<1.0 EU/ug
Formulation
Filtered(0.4 μm)andfrozenfrom0.3 –0.7 mg/mLsolutionin0.05 MAcetatebufferpH=4.0 containing5%oftrehalose.
Reconstitution
Defrostatambienttemperature
Applications
Westernblotting,ELISA
Shipping
Onice.Uponreceipt,storetheproductatthetemperaturerecommended below.
Storage/Expiration
Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cforthree days.
QualityControlTest
BCAtodeterminequantityoftheprotein.
SDSPAGEtodeterminepurityoftheprotein.
LALtodeterminequantityofendotoxin.
Note
Thisproductisintendedforresearchuseonly.TheCertificateofAnalysisondemand.