Type

Recombinant

Description

Neuregulin/He­regulinisafamilyofstructurallyrelatedpolypeptidegrowthfactorsderivedfromalternativelysplicedgenes(NRG1,NRG2,NRG3andNRG4).Todate,thereareover14 solubleandtransmembraneproteinsderivedfromtheNRG1gene.ProteolyticprocessingoftheextracellulardomainofthetransmembraneNRG1isoformsreleasessolublegrowthfactors.HRG1-β1containsanIgdomainandanEGF-likedomain;thelatterisnecessaryfordirectbindingtoreceptortyrosinekinaseserb3anderb4.Thisbindinginduceserb3anderb4heterodimerizationwitherb2,stimulatingintrinsickinaseactivitythatleadstotyrosinephosphorylation.AlthoughHRG1-β1"s BIOLOGicaleffectsarestillunclear,ithasbeenfoundtopromotemotilityandinvasivenessofbreastcancercells,whichmayalsoinvolveup-regulationofexpressionandfunctionoftheautocrinemotility-promotingfactor(AMF).RecombinantHumanHeregulinβ-1(HRG1-B1)isa7.5 kDapolypeptideconsistingofonlytheEGFdomainofheregulinβ-1(65 aminoacidresidues).

AminoAcidSequence

SHLVKCAEKEKTFCVNGGECFMVKDLSNPSRYLCKCPNEFTGDRCQNYVMASFYKHLGIEFMEAE

Source

E.coli

Purity

98%

BiologicalActivity

TheED₅₀ wasdeterminedbythedose-dependentstimulationoftheproliferationofhumanMCF-7 cellsis≤0.5 ng/ml,correspondingtoaspecificactivityof≥2×10⁶ units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C