Type

Recombinant

Description

HGFisapotent,mesenchymally-derivedmitogenformatureparenchymalhepatocytes,andactsasagrowthfactorforabroadspectrumoftissuesandcelltypes.HGFsignalsthroughatransmembranetyrosinekinasereceptorknownasMET.ActivitiesofHGFincludetheinductionofcellproliferation,motility,morphogenesis,inhibitionofcellgrowth,andenhancementofneuronsurvival.HGFisacrucialmitogenforliverregenerationprocesses,especiallyafterpartialhepatectomyandotherliverinjuries.HumanandmurineHGFarecross-reactive.MurineHGFisexpressedasalinear,polypeptide-precursorglycoproteincontaining696 aminoacidresidues.ProteolyticprocessingofthisprecursorgeneratestheBIOLOGicallyactiveheterodimericformofHGF,whichconsistsoftwopolypeptidechains(α-chainandβ-chain)heldtogetherbyasingledisulfidebondresultinginformationofabiologicallyactiveheterodimer.Theα-chainconsistsof463 aminoacidresiduesandfourkringledomains.Theβ-chainconsistsof233 aminoacidresidues.RecombinantMurineHGFisa79.3 kDapolypeptideconsistingof696 aminoacidresidues.

Source

(BTI-Tn-5B1-4)Hi-5Insectcells*

Purity

95%

BiologicalActivity

Determinedbythedose-dependentstimulationoftheproliferationofmouseIMCD3cellsusingaconcentrationrangeof10–20 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Note:Slowtodissolve.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C