Type

Recombinant

Description

Total142 AA.MW:15.97 kDa(calculated).UniProtKBacc.no.P05413.N-TerminalHis-tag,10 extraAA(highlighted).

AminoAcidSequence

MKHHHHHHASVDAFLGTWKLVDSKNFDDYMKSLGVGFATRQVASMTKPTTIIEKNGDILTLKTHSTFKNTEISFKLGVEFDETTADDRKVKSIVTLDGGKLVHLQKWDGQETTLVRELIDGKLILTLTHGTAVCTRTYEKEA

Source

E.coli

SDS-PAGEGel

14%SDS-PAGEseparationofHumanFABP3
1. M.W.Marker –14,21,31,45,66,97 kDa
2.reducedandboiledsample,5μg/lane
3.non-reducedandnon-boiledsample,5μg/lane

Formulation

Filtered(0,4 μm)andlyophilizedin0.5 mg/mLin20mMTRIS,50mMNaCl,pH 7.5

Reconstitution

Adddeionizedwatertoprepareaworkingstocksolutionof0.5 mg/mLandletthelyophilizedpelletdissolvecompletely.Filtersterilizeyourculturemedia/workingsolutionscontainingthisnon-sterileproductbeforeusingincellculture.

Applications

Westernblotting

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.