Type

Recombinant

Description

HCC-1 isaCCchemokinethatsignalsthroughtheCCR1receptorandchemoattractsbloodmonocytes.Itissecretedbyvarioustissues,includingskeletalmuscle,heart,spleen,liverandbonemarrow.MatureHCC-1 isfoundinfourdifferentforms,whicharedistinguishedbydifferentialN-terminaltrucationandcontain74,72,71,or66 aminoacidresidues.RecombinantHumanHCC-1 isan8.4 kDaproteinconsistingof72 aminoacids,includingthefourhighlyconservedresiduespresentinCCchemokines.

AminoAcidSequence

TESSSRGPYHPSECCFTYTTYKIPRQRIMDYYETNSQCSKPGIVFITKRGHSVCTNPSDKWVQDYIKDMKEN

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanmonocytesusingaconcentrationrangeof5.0–20.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C