Type

Recombinant

Description

AllthreeisoformsofGROareCXCchemokinesthatcansignalthroughtheCXCR1orCXCR2receptors.TheGROproteinschemoattractandactivateneutrophilsandbasophils.RecombinanthumanGRO-γisa7.9 kDaproteinconsistingof73 aminoacidsincludingthe‘ELR’motifcommontotheCXCchemokinefamilythatbindtoCXCR1or CXCR2.

AminoAcidSequence

ASVVTELRCQCLQTLQGIHLKNIQSVNVRSPGPHCAQTEVIATLKNGKKACLNPASPMVQKIIEKILNKGSTN

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattract293 transfectedCXCR2cellsusingaconcentrationrangeof10–100 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C