Type
Recombinant
Description
Gremlin-1(isoform-1)belongstoagroupofdiffusIBLeproteinsthatbindtoligandsoftheTGF-βfamilyandregulatetheiractivitybyinhibitingtheiraccesstosignalingreceptors.TheinterplaybetweenTGF-βligandsandtheirnaturalantagoNISTshasmajorBIOLOGicalsignificanceduringdevelopmentprocesses,inwhichcellularresponsecanvaryconsiderablydependinguponthelocalconcentrationofthesignalingmolecule.Gremlin-1 ishighlyexpressedinthesmallintestine,fetalbrain,andcolon;andisexpressedatlowerlevelsinthebrain,prostate,pancreas,andinskeletalmuscle.Gremlin-1 regulatesmultiplefunctionsinearlydevelopmentbyspecificallybindingto,andinhibitingthefunctionof,BMP-2, –4,and –7.Italsoplaysaroleincarcinogenesisandkidneybranchingmorphogenesis.RecombinantHumanGremlin-1 isa18.3 kDaproteincontaining160 aminoacidresidues.
AminoAcidSequence
KKKGSQGAIPPPDKAQHNDSEQTQSPQQPGSRNRGRGQGRGTAMPGEEVLESSQEALHVTERKYLKRDWCKTQPLKQTIHEEGCNSRTIINRFCYGQCNSFYIPRHIRKEEGSFQSCSFCKPKKFTTMMVTLNCPELQPPTKKKRVTRVKQCRCISIDLD
Source
ChineseHamsterOvaryCells(CHO)
Purity
90%
BiologicalActivity
DeterminedbyitsABIlitytoinhibitBMP-4 inducedalkalinephosphataseproductionbyATDC-5 chondrogeniccells.TheED50 forthiseffectis0.07–0.11µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.
Storage/Expiration
–20°C
