Type

Recombinant

Description

GranzymeBisacysteineproteasefoundinthecytoplasmicgranulesofcytolyticTlymphocytes(CTL)andnaturalkiller(NK)cells.GranzymeBisrequiredfortheinductionoftargetcelllysis,whichoccursaspartofcell-mediatedimmuneresponses,andcanactivateapoptosisintargetcellsbybothcaspase-dependentandcaspase-independentmechanisms.ProteolyticcleavageofsubstratesbyGranzymeBtakesplaceprimarilyafterasparticacidresidues.RecombinantMurineGranzymeBisaglycosylated227 aminoacidprotein,comprisingthematureactiveportionofthemurineGranzymeBprecursor.Theapparentmolecularweightis28.9 kDabymassspectrometry.

AminoAcidSequence

IIGGHEVKPHSRPYMALLSIKDQQPEAICGGFLIREDFVLTAAHCEGSIINVTLGAHNIKEQEKTQQVIPMVKCIPHPDYNPKTFSNDIMLLKLKSKAKRTRAVRPLNLPRRNVNVKPGDVCYVAGWGRMAPMGKYSNTLQEVELTVQKDRECESYFKNRYNKTNQICAGDPKTKRASFRGDSGGPLVCKKVAAGIVSYGYKDGSPPRAFTKVSSFLSWIKKTMKSS

Source

Baculovirus

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytocleaveasyntheticchromogenticGranzymeBsubstrate.Theexpectedspecificactivity,whenusingtheAc-IEPD-pNAsubstrateat25οC,isgreaterthan750 nM/minperμgofenzyme.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C

Storage/Expiration

 –20°C