Type

Recombinant

Description

GranulocyteColonyStimulatingFactorHumanRecombinantproducedinE.coliisasingle,glycosylated,polypeptidechaincontaining174 aminoacidsandhavingamolecularmassof20 KD.G-CSFispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP

Source

ChineseHamsterOvaryCells(CHO)

Purity

Greaterthan97.0%asdeterminedby:-AnalysisbyRP-HPLC.-AnalysisbySDS-PAGE.

BIOLOGicalActivity

TheED50,calculatedbythedose-dependantproliferationofmurineNFS-60 indicatorcells(measuredby3H-thymidineuptake)is<0.7 ng/ml,correspondingtoaSpecificActivityof1.27×108 IU/mg.

Formulation

G-CSFwaslyophilizedfromaconcentrated(1mg/ml)solutioncontainingPhosphate-BufferedSaline,pH 7.4

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedGranulocyteColonyStimulatingFactoralthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionG-CSFshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.