Type
Recombinant
Description
GM-CSFisahematopoieticgrowthfactorthatstimulatesthedevelopmentofneutrophilsandmacrophages,andpromotestheproliferationanddevelopmentofearlyerythroidmegakaryocyticandeosinophilicProgenitorcells.Itisproducedinendothelialcells,monocytes,fibroblastsandT-lymphocytes.GM-CSFinhibitsneutrophilmigrationandenhancesthefunctionalactivityofthematureend-cells.Thehumanandmurinemoleculesarespecies-specificandexhibitnocross-speciesreactivity.RecombinantMurineGM-CSFisa14.2 kDaglobularproteinconsistingof125 aminoacidresidues.
AminoAcidSequence
MAPTRSPITVTRPWKHVEAIKEALNLLDDMPVTLNEEVEVVSNEFSFKKLTCVQTRLKIFEQGLRGNFTKLKGALNMTASYYQTYCPPTPETDCETQVTTYADFIDSLKTFLTDIPFECKKPVQK
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofmurineFDC-P1cellsis≤0.05 ng/ml,correspondingtoaspecificactivityof≥2×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
