Type

Recombinant

Description

GM-CSFisahematopoieticgrowthfactorthatstimulatesthedevelopmentofneutrophilsandmacrophages,andpromotestheproliferationanddevelopmentofearlyerythroidmegakaryocyticandeosinophilicProgenitorcells.Itisproducedinendothelialcells,monocytes,fibroblastsandT-lymphocytes.GM-CSFinhibitsneutrophilmigrationandenhancesthefunctionalactivityofthematureend-cells.Thehumanandmurinemoleculesarespecies-specificandexhibitnocross-speciesreactivity.RecombinantHumanGM-CSFisa14.6 kDaglobularproteinconsistingof128 aminoacids,containingtwointramoleculardisulfidebondsandtwopotentialN-linkedglycosylation si­tes.

AminoAcidSequence

MAPARSPSPSTQPWEHVNAIQEARRLLNLSRDTAAEMNETVEVISEMFDLQEPTCLQTRLELYKQGLRGSLTKLKGPLTMMASHYKQHCPPTPETSCATQIITFESFKENLKDFLLVIPFDCWEPVQE

Source

E.coli

Purity

98%

BIOLOGicalActivity

TheED₅₀ asdeterminedbythedose-dependentstimulationoftheproliferationofhumanTF-1 cellsis≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×10⁷ units/mg.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C