Description

GranulocyteMacrophageColonyStimulatingFactorHumanRecombinantproducedinE.coliisasingle,non-glycosylated,polypeptidechaincontaining127 aminoacidsandhavingamolecularmassof14477 Dalton.GM-CSFispurifiedbyproprietarychromatographictechniques.

AminoAcidSequence

ThesequenceofthefirstfiveN-terminalaminoacidswasdeterminedandwasfoundtobeAla-Pro-Ala-Arg-Ser.N-terminalmethioninehasbeencompletelyremovedenzymatically.

Source

E.coli

Purity

Greaterthan98.0%asdeterminedby:(a)AnalysisbyRP-HPLC.(b)AnalysisbySDS-PAGE

BIOLOGicalActivity

TheED50asdeterminedbythedose-dependantstimulationoftheproliferationofhumanTF-1 cells(humanerythroleukemicindicatorcellline)is<0.1 ng/ml,correspondingtoaSpecificActivityof11.1×106 IU/mg.

Formulation

GM-CSFwaslyophilizedafterextensivedialysisagainst2 mMsodiumphosphatebufferpH=7.4±0.1.

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

LyophilizedGranulocyteMacrophageColonyStimulatingFactoralthoughstableatroomtemperaturefor3 weeks,shouldbestoreddesiccatedbelow –18°C.UponreconstitutionGMCSFshouldbestoredat4°Cbetween2–7daysandforfutureusebelow –18°C.Forlongtermstorageitisrecommendedtoaddacarrierprotein(0.1%HSAorBSA).Pleasepreventfreeze-thawcycles.

PhysicalAppearance

Sterilefilteredwhitelyophilized(freeze-dried)powder.