Type

Recombinant

Description

GDNFisadisulfide-linked,homodimericneurotrophicfactorstructurallyrelatedtoArtemin,NeurturinandPersephin.Theseproteinsbelongtothecysteine-knotsuperfamilyofgrowthfactorsthatassumestabledimericproteinstructures.GDNFsignalsthroughamulticomponentreceptorsystem,composedofaRETandoneofthefourGFRα(α1-α4)receptors.GDNFspecificallypromotesdopamineuptakeandsurvival,andmorphologicaldifferentiationofmidbrainneurons.UsingaParkinson’s diseasemousemodel,GDNFhasbeenshowntoimproveconditionssuchasbradykinesia,rigidity,andposturalinstABIlity.ThefunctionalratGDNFligandisadisulfide-linkedhomodimerconsistingoftwo15 kDapolypeptidechainscalledmonomers.Eachmonomercontainssevenconservedcysteineresidues,includingCys-101,whichisusedforinter-chaindisulfidebridging,andothersthatareinvolvedintheintramolecularringformationknownasthecysteineknotconfiguration.ThecalculatedmolecularweightofRecombinantRatGDNFis30.0 kDa.

AminoAcidSequence

MSPDKQAAALPRRERNRQAAAASPENSRGKGRRGQRGKNRGCVLTAIHLNVTDLGLGYETKEELIFRYCSGSCEAAETMYDKILKNLSRSRRLTSDKVGQACCRPVAFDDDLSFLDDSLVYHILRKHSAKRCGCI

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsabilitytostimulatetheproliferationofratC6 cells.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C