Type
Recombinant
Description
GDF-7 belongstotheTGF-βsuperfamilyofgrowthanddifferentiationfactors.Itisexpressedselectivelybyroofplatecellsthatarelocatedinthedevelopingembryoniccentralnervoussystem,andhasbeenshowntoinfluencetheneuronalidentityofcellswithinthecentralnervoussystem.GDF-7 hasalsobeenimplicatedintheformation,maintenance,andrepairofcertaincartilageandligamenttissues.RecombinantHumanGDF-7 isa28.0 kDadisulfide-linkedhomodimercontainingtwo129 aminoacidresidues.
AminoAcidSequence
TALAGTRTAQGSGGGAGRGHGRRGRSRCSRKPLHVDFKELGWDDWIIAPLDYEAYHCEGLCDFPLRSHLEPTNHAIIQTLLNSMAPDAAPASCCVPARLSPISILYIDAANNVVYKQYEDMVVEACGCR
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinhibitinducedalkalinephosphataseproductionbyATDC-5 chondrogenic cells.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
