Type
Recombinant
Description
GDF-5 isexpressedinlongbonesduringembryonicdevelopmentandpostnatallyinarticularcartilage.MutationsintheGDF-5 genehavebeenimplicatedinHunter-ThompsontypedwarfismandinGrebeSyndrome,whichischaracterizedbyshortstature,extradigits,andshortanddeformedextremities.ThematureandfunctionalformofGDF-5 isahomodimeroftwo120 aminoacidpolypeptidechains(monomers)linkedbyasingledisulfidebond.EachGDF-5 monomerisexpressedastheC-terminalpartofaprecursorpolypeptide,whichalsocontainsa27 aminoacidsignalpeptideanda348 aminoacidpropeptide.Thisprecursorundergoesintracellulardimerization,anduponsecretionitisprocessedbyafurin-typeprotease.RecombinantMurineGDF-5 isa27.0 kDahomodimericdisulfide-linkedproteinconsistingoftwo120 aminoacidpolypeptidechains.
AminoAcidSequence
APLANRQGKRPSKNLKARCSRKALHVNFKDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyATDC-5 cells. TheexpectedED50forthiseffectis1.0–2.0 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
