Type
Recombinant
Description
GDF-5 isexpressedinlongbonesduringembryonicdevelopmentandpostnatallyinarticularcartilage.MutationsintheGDF-5 genehavebeenimplicatedinHunter-ThompsontypedwarfismandinGrebeSyndrome,whichischaracterizedbyshortstature,extradigits,andshortanddeformedextremities.ThematureandfunctionalformofGDF-5 isahomodimeroftwo120 amino-acidpolypeptidechain(monomers)linkedbyasingledisulfidebond.EachGDF-5 monomerisexpressedastheC-terminalpartofaprecursorpolypeptide,whichalsocontainsa27 aminoacidsignalpeptideanda354 aminoacidpropeptide.Thisprecursorundergoesintracellulardimerization,anduponsecretionitisprocessedbyafurin-typeprotease.RecombinantHumanGDF-5 isa27.0 kDahomodimericdisulfide-linkedproteinconsistingoftwo120 aminoacidpolypeptidechains.License:ThisproductisproducedsolelybyPeproTechInc.underalicensefromBiopharmGmbH,Germany.BiopharmGmbHisnotresponsIBLeforthequalityorfitnessforuseofthisproduct.Itmaybeusedonlyforresearchpurposesandmayneitherbeusedinhumansnorforveterinarypurposes.Thisproductmaynotbeusedforcommercialpurposes.
AminoAcidSequence
APSATRQGKRPSKNLKARCSRKALHVNFKAPSATRQGKRPSKNLKARCSRKALHVNFKDMGWDDMGWDDWIIAPLEYEAFHCEGLCEFPLRSHLEPTNHAVIQTLMNSMDPESTPPTCCVPTRLSPISILFIDSANNVVYKQYEDMVVESCGCR
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 wasdeterminedbyitsABIlitytoinducealkalinephosphataseproductionbyATDC-5 chondrogeniccellsis1.0–2.0 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.ReconstituteinwatercontainingBSA(50ugofBSAper1ugofprotein)toaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
