Type

Recombinant

Description

GDF-3 isamemberoftheTGF-βsuperfamilyofgrowthanddifferentiationfactors,andishighlyhomologoustoGDF-9. UnlikemostTGF-βfamilymembers,GDF-3 andGDF-9 arenotdisulfide-linkeddimers.GDF-3 isexpressedinadultbonemarrow,spleen,thymus,andADIposetissue.TheexpressionofGDF-3 isupregulatedinhigh-fat-fedwild-typeFABP4/aP2nullmiceandwasassociatedwithobesity,butnotwiththerelatedhyperglycemia/hy­perinsulinemiathatcharacterizesType2 diabetes.RecombinantHumanGDF-3 isa26.0 kDanon-disulfide-linkedhomodimercontainingtwo114 aminoacidpolypeptidechains.

AminoAcidSequence

AAIPVPKLSCKNLCHRHQLFINFRDLGWHKWIIAPKGFMANYCHGECPFSLTISLNSSNYAFMQALMHAVDPEIPQAVCIPTKLSPISMLYQDNNDNVILRHYEDMVVDECGCG

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytoinhibitinducedalkalinephosphataseproductionbyATDC-5 chondrogeniccells.TheED₅₀ forthiseffectis100–150 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C