Type
Recombinant
Description
GDF-11 isamyostatin-homologousproteinthatactsasaninhibitorofnervetissuegrowth.GDF-11 hasbeenshowntosuppressneurogenesisthroughamyostatin-likepathway,whichinvolvesthearrestoftheProgenitorcellcycleintheG1phase.SimilaritiesbetweenmyostatinandGDF-11,whichare90%identicalintheiraminoacidsequence,suggestthattheregulatorymechanismsresponsIBLeformaintainingpropertissuesizeduringneuralandmusculardevelopmentmightbethesame.RecombinantHumanGDF-11 isa25.0 kDadisulfide-linkedhomodimercontainingtwo109 aminoacidpolypeptidechains.Itishighlyhomologoustomyostatin/GDF-8,sharing90%aminoacidsequenceidentity.
AminoAcidSequence
NLGLDCDEHSSESRCCRYPLTVDFEAFGWDWIIAPKRYKANYCSGQCEYMFMQKYPHTHLVQQANPRGSAGPCCTPTKMSPINMLYFNDKQQIIYGKIPGMVVDRCGCS
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50 wasdeterminedbyitsABIlitytoinhibitinducedalkalinephosphataseproductionbyATDC-5 chondrogeniccells.TheexpectedED50 forthiseffectis0.08–0.10 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Note:KeeppHbelow5.0. Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C
Storage/Expiration
–20°C
