Type
Recombinant
Description
G-CSFisahematopoieticgrowthfactorthatstimulatesthedevelopmentofcommittedProgenitorcellstoneutrophilsandenhancesthefunctionalactivitiesofthematureend-cell.Itisproducedinresponsetospecificstimulationbyavarietyofcellsincludingmacrophages,fibroblasts,endothelialcellsandbonemarrowstroma.G-CSFisbeingusedclinicallytofacilitatehematopoieticrecoveryafterbonemarrowtransplantation. HumanandmurineG-CSFarecross-speciesreactive.RecombinanthumanG-CSFisan18.7 kDaproteinconsistingof174 aminoacidresidues.
AminoAcidSequence
TPLGPASSLPQSFLLKCLEQVRKIQGDGAALQEKLCATYKLCHPEELVLLGHSLGIPWAPLSSCPSQALQLAGCLSQLHSGLFLYQGLLQALEGISPELGPTLDTLQLDVADFATTIWQQMEELGMAPALQPTQGAMPAFASAFQRRAGGVLVASHLQSFLEVSYRVLRHLAQP
Source
E.coli
Purity
98%
BIOLOGicalActivity
TheED50asdeterminedbythedose-dependentstimulationoftheproliferationofmurineNFS-60 cellsis≤0.1 ng/ml,correspondingtoaspecificactivityof≥1×107units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C