Type

Recombinant

Description

CXCL6,alsoknownasGCP-2 inhumans,isaconnectivetissue-derivedCXCchemokinethatcansignalthroughtheCXCR1andCXCR2receptors.HumanGCP-2,whichiscross-reactivewithmurinecells,selectivelyattractsneutrophils,andhasalsobeenshowntoexertanti-angiogenicactivity.RecombinantHumanGCP-2 isa7.9 kDaproteincontaining72 aminoacidresidues.

AminoAcidSequence

VLTELRCTCLRVTLRVNPKTIGKLQVFPAGPQCSKVEVVASLKNGKQVCLDPEAPFLKKVIQKILDSGNKKN

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanneutrophilsusingaconcentrationrangeof10.0–50.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.

Storage/Expiration

 –20°C