Type

Recombinant

Description

Total175 AA.MW:19.5 kDa(calculated).UniProtKBacc.no.Q9NS71(Asn35-Asn199).N-terminalHis-tag(10 extraAA).ProteinidentityconfirmedbyLC-MS/MS.

AminoAcidSequence

MKHHHHHHASNYNINVNDDNNNAGSGQQSVSVNNEHNVANVDNNNGWDSWNSIWDYGNGFAATRLFQKKTCIVHKMNKEVMPSIQSLDALVKEKKLQGKGPGGPPPKGLMYSVNPNKVDDLSKFGKNIANMCRGIPTYMAEEMQEASLFFYSGTCYTTSVLWIVDISFCGDTVEN

Source

E.coli

Purity

Purityasdeterminedbydensitometricimageanalysis: >90%

SDS-PAGEGel

14%SDS-PAGEseparationofHuGKN1:
1. M.W.Marker –97,66,45,31,21,14 kDa
2.reducedandheatedsample,2.5μg/lane
3.non-reducedandnon-heatedsample,2.5μg/lane

Endotoxin

<1.0 EU/ug

Formulation

Filtered(0.4 μm)andlyophilizedfrom0.5 mg/mLin20mMTrisbuffer,50 mMNaCl,pH 8.6

Reconstitution

Add200µlofdeionizedwatertoprepareaworkingstocksolutionofapproximately0.5 mg/mlandletthelyophilizedpelletdissolvecompletely.Productisnotsterile!Pleasefiltertheproductbyanappropriatesterilefilterbeforeusingitinthecellculture.

Applications

Westernblotting,ELISA

Shipping

Atambienttemperature.Uponreceipt,storetheproductatthetemperaturerecommended below.

Storage/Expiration

Storethelyophilizedproteinat –80°C.Lyophilizedproteinremainsstableuntiltheexpirydatewhenstoredat –80°C.Aliquotreconstitutedproteintoavoidrepeatedfreezing/thawingcyclesandstoreat –80°Cforlongtermstorage.Reconstitutedproteincanbestoredat4 °Cfora week.

QualityControlTest

BCAtodeterminequantityoftheprotein.

SDSPAGEtodeterminepurityoftheprotein.

LALtodeterminequantityofendotoxin.

Note

Thisproductisintendedforresearchuseonly.