Type
Recombinant
Description
Growthanddifferentiationfactor-associatedserumprotein-1(GASP-1)isasecretedinhibitoryTGF-βbindingproteinthatcontainsmultipleproteaseinhibitorstructuraldomains.Itisexpressedprimarilyintheovary,testis,andbrain,andcanactasapotentsolubleinhibitorofmyostatinandGDF-11,butnotActivinA.TheGASP-1 geneencodesa571 aminoacidproteinthatcontainsa29 aminoacidsecretionsignalsequence,andmultipleidentifiablestructuralfeatures,includingaWAPdomain,afollistatin/Kazaldomain,animmunoglobulindomain,twotandemKunitzdomains,andanetrindomain.RecombinantHumanGASP-1 isa542 aminoacidproteinthatmigratesatanapparentmolecularweightofapproximately55–66kDabySDS-PAGEanalysisundernon-reducingconditions.ThecalculatedmolecularweightofRecombinantHumanGASP-1 is59.9 kDa.
AminoAcidSequence
LPPIRYSHAGICPNDMNPNLWVDAQSTCRRECETDQECETYEKCCPNVCGTKSCVAARYMDVKGKKGPVGMPKEATCDHFMCLQQGSECDIWDGQPVCKCKDRCEKEPSFTCASDGLTYYNRCYMDAEACSKGITLAVVTCRYHFTWPNTSPPPPETTMHPTTASPETPELDMAAPALLNNPVHQSVTMGETVSFLCDVVGRPRPEITWEKQLEDRENVVMRPNHVRGNVVVTNIAQLVIYNAQLQDAGIYTCTARNVAGVLRADFPLSVVRGHQAAATSESSPNGTAFPAAECLKPPDSEDCGEEQTRWHFDAQANNCLTFTFGHCHRNLNHFETYEACMLACMSGPLAACSLPALQGPCKAYAPRWAYNSQTGQCQSFVYGGCEGNGNNFESREACEESCPFPRGNQRCRACKPRQKLVTSFCRSDFVILGRVSELTEEPDSGRALVTVDEVLKDEKMGLKFLGQEPLEVTLLHVDWACPCPNVTVSEMPLIIMGEVDGGMAMLRPDSFVGASSARRVRKLREVMHKKTCDVLKEFLGLH
Source
ChineseHamsterOvaryCells(CHO)
Purity
95%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinhibithumanMyostatin(GDF-8)activityinMCP-11 cells.TheED50 forthiseffectis0.0025–0.0040 µg/mlinthepresenceof5ng/mlofhumanMyostatin(GDF-8).
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
