Type

Recombinant

Description

Lectins,ofeitherplantoranimalorigin,arecarbohydrate-bindingproteinsthatinteractwithglycoproteinandglycolipidsonthesurfaceofanimalcells.TheGalectinsarelectinsthatrecognizeandinteractwithβ-galactosidemoieties.Galectin-1 isananimallectinthathasbeenshowntointeractwithCD3,CD4,andCD45.ItinducesapoptosisofactivatedT-cellsandT-leukemiacelllines,andinhibitstheproteinphosphataseactivityofCD45.RecombinantHumanGalectin-1 isa14.5 kDaproteincontaining134 aminoacidresidues.

AminoAcidSequence

ACGLVASNLNLKPGECLRVRGEVAPDAKSFVLNLGKDSNNLCLHFNPRFNAHGDANTIVCNSKDGGAWGTEQREAVFPFQPGSVAEVCITFDQANLTVKLPDGYEFKFPNRLNLEAINYMAADGDFKIKCVAFD

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanbloodmonocytes.Chemotacticactivitywasobservedataconcentrationof2.5 µg/mlwithapeakresponseobtainedat250µg/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C