Type
Recombinant
Description
ThegAcrp30variantisanaturallyoccurringglobularproteinobtainedbyproteolyticprocessingofADIponectin.Adiponectinisproducedandsecretedexclusivelybyadipocytes,andisarelativelyabundantplasmaprotein,accountingforupto0.05%oftotalserumprotein.LikeAdiponectin,Acrp30iscapableofdecreasinghyperglycemiaandreversinginsulinresistance.Additionally,gAcrp30hasbeenshowntobeanimportantfactorinpromotingfatlossbysignalingmuscletoabsorbandburnFree-FattyAcids(FFAs).ThesignalingreceptorsforadiponectinandgAcrp30haverecentlybeenidentifiedandnamedAdipoR1andAdipoR2.AdipoR2ispredominantlyexpressedintheliver.ThisnaturallyoccurringvariantofhumangAcrp30/Adipoleanisan18.1 kDaprotein,containing14 extraaminoacidsextraattheN-terminusofhumangAcrp30/Adipolean.
AminoAcidSequence
PGAEGPRGFPGIQGRKGEPGEGAYVYRSAFSVGLETYVTIPNMPIRFTKIFYNQQNHYDGSTGKFHCNIPGLYYFAYHITVYMKDVKVSLFKKDKAMLFTYDQYQENNVDQASGSVLLHLEVGDQVWLQVYGEGERNGLYADNDNDSTFTGFLLYHDTN
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyacytotoxicityassayusingM1cells.TheexpectedED50 forthiseffectis0.5–1.0 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
