Type
Recombinant
Description
gAcrp30isanaturallyoccurringglobularprotein,obtainedbyproteolyticprocessingofADIponectin.Adiponectinisproducedandsecretedexclusivelybyadipocytesandisarelativelyabundantplasmaprotein,accountingforupto0.05%oftotalserumprotein.LikeAdiponectin,gAcrp30iscapableofdecreasinghyperglycemiaandreversinginsulinresistance.Additionally,gAcrp30hasbeenshowntobeanimportantfactorinpromotingfatlossbysignalingmuscletoabsorbandburnFree-FattyAcids(FFAs).ThesignalingreceptorsforadiponectinandgAcrp30haverecentlybeenidentifiedandnamesAdipoR1andAdipoR2.AdipoR2ispredominantlyexpressedintheliver. RecombinantmurinegAcrp30isa16.6 kDaproteinconsistingof145 aminoacidresidues.
AminoAcidSequence
MKGEPGEAAYMYRSAFSVGLETRVTVPNVPIRFTKIFYNQQNHYDGSTGKFYCNIPGLYYFSYHITVYMKDVKVSLFKKDKAVLFTYDQYQEKNVDQASGSVLLHLEVGDQVWLQVYGDGDHNGLYADNVNDSTFTGFLLYHDTN
Source
E.coli
Purity
98%
BIOLOGicalActivity
DeterminedbyitsABIlitytoinhibittheproliferationofmurinemyeloidcelllinesM1.TheED50forthiseffectis≤15 µg/ml.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
