Type

Recombinant

Description

FractalkineisaCX3CLchemokinethatsignalsthroughtheCX3CR1receptor.Fractalkinehasbeenshowntochemoattractmonocytes,microgliacellsandNKcells.Fractalkineis,atthistime,theonlyCXC3Cchemokinethatcontainsthreeaminoacidresiduesbetweenthefirstandsecondcysteineresiduesofthechemokinedomain.TheFractalkinegeneencodesfora397 aminoacidprecursorproteincontaininga24 aminoacidsignalsequence,achemokinedomain,anda“mucin-likestalk”sequence,whichisfollowedbythetransmembranedomaincontainingapproximately20 aminoacids,andaC-terminalcytoplasmicdomain.Theextracellularchemokinedomaincontains76 aminoacidresidues,includingthefourconservedcysteineresiduesfoundinotherchemokines.RecombinantHumanFractalkineisan8.5 kDaproteincontaining76 aminoacidresidues,includingthefourconservedcysteineresiduespresentinCCchemokines.RecombinantRatFractalkineisan8.7 kDaproteincontaining76 aminoacidresidues,includingthefourconservedcysteineresiduespresentinCCchemokines.

AminoAcidSequence

QHLGMTKCNITCHKMTSPIPVTLLIHYQLNQESCGKRAIILETRQHRHFCADPKEKWVQDAMKHLDHQTAALTRNG

Source

E.coli

Purity

98%

BIOLOGicalActivity

DeterminedbyitsABIlitytochemoattracthumanmonocytesusingaconcentrationrangeof5.0–10.0 ng/ml.

Endotoxin

Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).

Reconstitution

Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat-20°Cto –80°C.

Storage/Expiration

 –20°C