Type
Recombinant
Description
Flt3-Ligandisagrowthfactorthatregulatesproliferationofearlyhematopoieticcells.Flt3-LigandbindstocellsexpressingthetyrosinekinasereceptorFlt3.Flt3-Ligand,byitselfdoesnotstimulateproliferationofearlyhematopoieticcells,butsynergizeswithotherCSFsandinterleukinstoinducegrowthanddifferentiation.UnlikeSCF,Flt3-Ligandexertsnoactivityonmastcells.MultipleisoformsofFlt3-Ligandhavebeenidentified.ThepredominantBIOLOGicallyactiveformisanchoredtothecellsurfaceastheextracellulardomainofatransmembraneprotein(209 a.a.).Themembrane-boundisoformcanbeproteolyticallycleavedtogenerateabiologicallyactivesolubleisoform.RecombinantHumanFlt3-Ligandisasoluble17.6 kDaproteinconsistingof155 aminoacidresidues.
AminoAcidSequence
MTPDCYFSHSPISSNFKVKFRELTDHLLKDYPVTVAVNLQDEKHCKALWSLFLAQRWIEQLKTVAGSKMQTLLEDVNTEIHFVTSCTFQPLPECLRFVQTNISHLLKDTCTQLLALKPCIGKACQNFSRCLEVQCQPDSSTLLPPRSPIALEATELPEPRPRQ
Source
E.coli
Purity
98%
BiologicalActivity
TheED50wasdeterminedbythedose-dependentstimulationoftheproliferationofhumanAML5cellsis≤1.0 ng/ml,correspondingtoaspecificactivityof≥1×106units/mg.
Endotoxin
Endotoxinlevelis<0.1 ng/μgofprotein(<1EU/μg).
Reconstitution
Centrifugethevialpriortoopening.Reconstituteinwatertoaconcentrationof0.1–1.0 mg/ml.Donotvortex.Thissolutioncanbestoredat2–8°Cforupto1 week.Forextendedstorage,itisrecommendedtofurtherdiluteinabuffercontainingacarrierprotein(example0.1%BSA)andstoreinworkingaliquotsat –20°Cto –80°C.
Storage/Expiration
–20°C
